Stilbene-based derivatives as potential inhibitors of trimethylamine (TMA)-lyase affect gut microbiota in coronary heart disease.

Coronary heart disease (CHD) is defined by atherosclerosis, which may result in stenosis or blockage of the arterial cavity, leading to ischemic cardiac diseases such as angina and myocardial infarction (MI). Accumulating evidence indicates that the gut microbiota play a critical role in the initiation and progression of CHD. The gut microbial metabolite trimethylamine N-oxide (TMAO) is intimately linked to the pathophysiology of CHD. The hepatic flavin-containing monooxygenases (FMOs) convert trimethylamine (TMA) to TMAO. As a result, it is critical to prevent TMA generation. Stilbenes could reduce cardiovascular disease mortality. Twelve stilbenes with inhibitory activity against TMA-lyase were compiled and evaluated in this study. Docking results showed Resveratroloside had the highest Vina score, indicating that it was the most active and might be employed as a lead molecule for further structural modification.

[Rapid identification for fruit drugs of rosaceae based on electrochemical oscillation reaction].

OBJECTIVE: To establish a rapid identification method for fruit drugs of Rosaceae, according to the influence of Chinese traditional medicine on the electrochemical oscillation reaction. METHODS: The experiments were carried out in H2SO4-Ce (SO4)2-CH2 (COOH)2-KBrO3 electrochemical oscillation system. The graphs and characteristic parameters which were formed by the disturbance of Rosaceous fruit drugs to the electrochemical oscillation reaction were studied. RESULTS: There were obvious distinction on the electrochemical fingerprints of five kinds of medicinal materials, and the characteristic parameters of oscillation life were significant different. CONCLUSION: The electrochemical fingerprints of five kinds of Rosaceous fruit drugs can be used to identify those medicinal materials. The method is simple, rapid, economic and reliable.

Poly (O-aminobenzoic acid) modified glassy carbon electrode for electrochemical detection of dopamine in the presence of ascorbic acid.

O-aminobenzoic acid (o-ABA) film is deposited on glassy carbon electrode (GCE) by electropolymerization in pH 7.0 phosphate buffer solution (PBS). The polymeric film shows an excellent electrocatalytical activity on the oxidation of dopamine (DA). Difference pulse voltammetry (DPV) was performed to determine DA in an excess of ascorbic acid (AA). The oxidation peak potentials of DA and AA recorded are 144 mV and -52 mV, respectively. In pH 7.0 PBS, the anodic peak current of DA increases linearly over two concentration intervals, viz., 1.0x10(-7)-1.0x10(-5) mol L(-1) and 1.0x10(-5) - 2.0x10(-4) mol L(-1), with correlation coefficient, 0.9966 and 0.9960, respectively. The relative standard deviation of 10 successive scans is 2.8 % for 1.0x10(-6) mol L(-1) DA and the recovery is 96 % - 101 %. The interference of AA and DOPAC with the determination of DA could be eliminated because of the very distinct attracting interaction between DA cations and the negatively poly (o-ABA) film in pH 7.0 PBS. The proposed method exhibits good recovery and reproducibility.